Try 7-AAD (7-amino-actinomycin D). Protocol and illustrations (including important fluorescence overlap compensation details) are on the Web at:
URL <http://nucleus.immunol.washington.edu/Research_facilities/Apps/7aad.html>
Dave Coder
dcoder@u.washington.edu
Begin forwarded message:
Date: Thu, 23 Jan 97 09:30:44 EST
From: "Adrian Moris" <Adrian_Moris@sratech.com>
Encoding: 11 Text
To: cyto-inbox
Subject: Live vs. Dead cells?
Dear Flow Folks:
A Co-worker and I are are wondering if there is a way to stain cells
for flow which will distinguish between live and dead cells when
co-stained with a PE conjugated Ab. Does anyone have such a protocol
they would share?
Thanks
Adrian Moris
SRA Technologies
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