[This message is converted from WPS-PLUS to ASCII] Dear colleagues, When performing FACS analysis following BrdU labeling, can anything be understood from the shape of the S phase profile? In control cells (HCT116 - Human colon tumor cells), we see a tight uniform profile of cells entering and exiting S phase - the classic U shape. In cells treated with compound, the S-phase is more diffuse and has lost a tiny bit of fluorescence as compared to control. Could this indicate variable rates of DNA synthesis or a block at the entry to G2/M or does it mean nothing at all. Thanks in advance for any responses. Diane Sharp DuPont Merck Pharmaceutical Company Wilmington, DE
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