Hi folks, I have a lab here that wants to sort sperm for PCR, ie. they're not interested in X-Y determination, just sorting single sperm into 96-well plates for amplification. I was about to suggest staining with Hoechst -342 (we have UV) but does anyone have a feel for (a) the overall feasibility of this idea, and (2) whether Hoechst or any other DNA stain will interfere with PCR? While I'm in Pine, does anyone have any tipes for sorting chromosomes on a 753? How bout when n is greater than 35? Looks like it's gonna be an interesting year....thanks for any leads you can provide. Steve ------------------------------------------------------------- Steve G. Hilliard This space available, Cell Analysis Facility email me for rates! University of Georgia -------------------------------------------------------------
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