Sperm sorting

From: Gary Durack (durack@ux1.cso.uiuc.edu)
Date: Wed Jan 15 1997 - 11:53:06 EST

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Steve

We have been using -342 to identify bovine sperm, then sorting into
microwell plates for PCR.  No problems seen with the -342 complicating the
PCR wizardry. 

Here are a few references from this work.

Lewin, H. A., K. Schmitt, R. Hubert, M. J. T. van Eijk and N. Arnheim.
1992.  Close linkage between bovine prolactin and BoLA-DRB3 genes:  genetic
mapping in cattle by single sperm typing.  Genomics 13:44-48.

Van Eijk, M. J. T., I. Russ and H. A. Lewin.  1993.  Order of bovine DRB3,
DYA and PRL determined by sperm typing.  Mammalian Genome 4:113-118.

Park, C., I Russ, Y. Da, and H. A. Lewin. 1995. Genetic mapping of F13A to
BTA23 by sperm typing: difference in recombination rate between bulls in
the DYA-PRL interval.  Genomics 27: 113-118.

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