Anti-human Perforin, clone deltaG9

BACKGROUND

Perforin has a key role in cell-mediated cytotoxicity. It is a cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells (reviewed in 1). CTLs are involved in eliminating virally infected cells, in anti-tumor immune responses, in allograft rejections, and in some autoimmune diseases. NK cells are important for tumor surveillance and destruction and are involved in allograft rejections. Cytotoxic cells release the contents of their cytotoxic granules, including perforin upon recognition of their target cell. In the presence of calcium, perforin forms transmembrane channels or pores in the membrane of the target cell leading to a cell death that resembles apoptosis. The ability to detect perforin-positive cells with specific antibody should be useful in identifying and understanding perforin-mediated reactions.

SPECIFICITY

Clone dG9 reacts with human perforin.(1) It does not cross-react with mouse perforin.(1) Purified granules from the human lymphoma cell line YT were used as immunogen.(1) Clone dG9 was initially characterized by immunoprecipitation and immunohistochemistry of frozen tissue sections.(1) The antibody is routinely tested by immunoprecipitation using YT cells, flow cytometric analysis using peripheral blood mononuclear cells (PBMCs), and immunohistochemistry of frozen tissue sections of spleen and lymphoma. The antibody stains scattered lymphocytes in red pulp of spleen, and scattered infiltrated lymphocytes in lymphoma.

USAGE

Applications include immunoprecipitation (1-2 µg/one million cells),(1) flow cytometry (titrate between 0.06-1 µg/1 x 10 million cells), indirect immunofluorescence microscopy of cytospins,(1) and immunohistochemistry of frozen tissue sections (1-2 µg/ml).(1,2,3 ) Clone dG9 is not recommended for western blot analysis or immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections.

Cat. No. Description Clone Size
65991A Purified anti-human Perforin deltaG9 0.1mg

For Research Use Only. Not For Diagnostic or Therapeutic Use.

REFERENCES

  1. Hameed, A., K.J. Olsen, L. Cheng, W.M. Fox, R.H. Hruban, and E.R. Podak. 1992. Immunohistochemical identification of cytotoxic lymphocytes using perforin monoclonal antibody. Am. J. Pathol. 140:1025-1030.
  2. Fox, W.J., A. Hameed, G. Hutchins, B.A. Reitz, W.A. Baumgartner, W.E. Beschorner, and R.H. Hruban. 1993. Perforin expression localizing cytotoxic lymphocytes in the intimas of coronary arteries with transplant-related accelerated arteriosclerosis. Human Pathol. 24:477-482.
  3. Hameed, A., E.R. Podack, W.M. Fox, R.W. Schafer, and M.E. Sherman. Detection of perforin in human peritoneal fluid T-lymphocytes. Acta Cytologica 40:401-407.

Related Products for Apoptosis Research:

* Please inquire for additional Bcl-2 antibodies and other Apoptosis reagents.

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