Apoptosis, a term that describes regulated cell death, is a fundamental feature of many biological processes including normal development, homeostasis, and disease. Changes in the plasma membrane of the cell surface are one of the earliest features of cells undergoing apoptosis. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner leaflet of the plasma membrane to the outer leaflet, thereby exposing PS to the external environment. Annexin V is a Ca2+-dependent phospholipid-binding protein that has a high affinity for PS, and is useful for identifying apoptotic cells with exposed PS. Since externalization of PS occurs earlier than nuclear changes associated with apoptosis, Annexin V Conjugates can identify cells in an earlier stage of apoptosis than assays based on DNA fragmentation.
Annexin V-FITC can be used in a bivariate analysis with Propidium Iodide (PI) to distinguish between cells undergoing apoptosis (PI negative) and those that are necrotic or dead (PI positive).
Annexin VPE is typically used in conjunction with a vital dye such as 7-Amino-actinomycin (7AAD, Cat. #34321X) to distinguish apoptotic cells (Annexin V-PE positive, 7-AAD negative) from necrotic cells (Annexin VPE positive, 7AAD positive).
PharMingen's Annexin V Conjugates are available in 100 and 200 test sizes and are supplied with complete protocols.
| Cat. No. | Description | Size |
| 65874X | Annexin V-FITC | 100 tests |
| 65874H | Annexin V-FITC | 200 tests |
| 65875X | Annexin V-PE | 100 tests |
| 65875H | Annexin V-PE | 200 tests |
For Research Use Only. Not For Diagnostic or Therapeutic Use.
Related Antibodies and Kits:
* Please inquire for additional Bcl-2 antibodies and other Apoptosis reagents.
ÝAPO-BRDU™ and APO-DIRECT™ are registered trademarks of Phoenix Flow Systems, San Diego, CA.
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