Re: Cryopreservation- Annexin V binding

Piotr Wilczek (PIOTR@serce.fcsd.gliwice.pl)
Tue, 16 Sep 1997 09:32:36 MET-DST

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> In my experience DMSO preservatives do indeed lead to Annexin-V
> positivity. This seems to be the case with both tumour FNA samples and
> trypsinised cell lines. The preservative I use is the standard freezing
> mixture, 10% DMSO in fetal calf serum.
>
> Eric P Miller
> Edinburgh Medical Oncology Unit
>
> "Wouldn't it be nice if hospitals and schools had all the money they
> needed and the army had to hold jumble sales to buy guns?"
>
Also in my experience with cryopreserrved tissue i have observed
about 30-40% of cells positiv to Annexin-V.What is your opinion about
the mechanism bay witch DMSO inicjate the inverse of cell membrane.
Is this really a apoptotic process. Could your recomend me some
materaials (publications) about this.

Piotr Wilczek, MSc
Foundation of Cardiac Surgery Development
tel.: (+48 32) 271-06-31 ext. 118, 131
fax: (+48 32) 271-38-00
piotr@serce.fcsd.gliwice.pl

Next message: David L. Haviland, Ph.D.: "Re: Flow cytometer location"
Previous message: Larry Arnold: "Cell surface receptor - Lost answer"
Maybe in reply to: Peter Kierulf: "Cryopreservation- Annexin V binding"