I wonder how many laboratories do the nuclear TdT stain
by flow cytometric method after membrane permeabilization
not by cytospin followed by fluorscence microscopic reading?
In case of flow cytometric method, which protocol for membrane
permeabilization you use?
Is the permeabilization reagent Triton-X, saponin, FACS Lysing Solution,
FACS Permeabilization Solution, Fix&Perm, or something special?
And, have you ever tried to compare the results between
flow cytometric method and cytospin method?
Any comments will be appreciated.
Thanks in advance.
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Chang-Seok Ki, M.D.
Dept. of Clinical Pathology
Samsung Medical Center
Seoul, Korea
Tel. 82-2-3410-2708
Fax. 82-2-3410-2719
E-mail. kcdol@samsung.co.kr
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