Kevin L. Holmes, Ph.D.
Head, Flow cytometry Unit
Office of the Scientific Director
Bldg. 7, Room 01
NIAID, NIH
Phone: 301-496-9071
FAX: 301-402-4532
Email: kholmes@atlas.niaid.nih.gov
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From:
KUKURUGA@medmail.med.umich.edu[SMTP:KUKURUGA@medmail.med.umich.edu]
Sent: Thursday, September 11, 1997 1:14PM
To: cyto-inbox
Subject: biotin-avidin sandwiches, anyone?
I've heard of a technique for amplifying signal of low density
antigens by
creating layers of avidin-biotin complex, thereby increasing the
available
biotin targets for the finally added fluorescent-conjugated avidin.
Anyone
know about this, and do you have references? I've searched, but I
guess
I can't come up with the proper key words.
Thanks.
MAK.
--- Mark A. KuKuruga, Managing Director University of Michigan Flow Cytometry kukuruga@medmail.med.umich.edu