Now, with the TdT assay, I've been running into difficulties determining
where the apoptosis cells actually are. I'm using the Boehringer Mannheim
"In Situ Cell Death Detection Kit, Fluorescein". However, the pamphlet is
not at all clear on how to analyze the data. What I have observed so far
is this: with negative control cells displayed on a logarithmic histogram
of FL-1, I get one large peak at around 10^1. With positive control
(camptothecin-treated) cells, that large peak is shifted slightly to the
left. Furthermore, the number of cells with high dUTP incorporation
increases.
My difficulty in interpretation lies in this: which is the significant
property of the data? The shift in the large peak to the right, or the
increase in high dUTP incorporation? In literature, either one or the
other seems to be used to evaluate degree of apoptosis. Does anybody have
any suggestions about interpretation of data from the TdT assay?
Thanks,
Ryan.
_/ \__/ \__/ \__/ \__/ \__/ \__/ \__/rhung@vcn.bc.ca__/ \__/ \__/
\_Apoptosis=programmed cell death/ \__/ \rwhung@unixg.ubc.ca_/ \__/ \__
_/ --you can't live without it!/ \__/ \http://www.vcn.bc.ca/people/rhung
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