I have an investigator who is analyzing murine embryonic fibroblasts
(MEF) for cell cycle perturbations based on retrovirally infected cell cycle
inhibitory proteins. We have regularly observed what appears to be a true
tetraplod population, ie., exhibiting singlet cell properties based on pulse
processing (FACScan FL-2A vs. FL-2W), PI nucelear isolation prep. We see
this in both normal control and experimental preps.
Is a tetraploid condition a known feature of MEF biology? These are
isolated from fetuses and placed directly into primary culture. We have
seen it often enough that I want to believe it, but it is controversial.
Thanks for the input....regards, Dave G.
Dave Gebhard
Director FACS/ Oligonucelotide Synthesis Facilities
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, New York 10461
718-430-2724/ 3573