We received a specimen of CML for flow and HLA-phenotyping.
The peripheral blood count was 280,000 WBC/mm3. The flow went fine but
the traditional HLA determination for class I was not feasible
because of the high leukemic cells in circulation. As B-cells have also
class i receptor on their surface, I decided to go ahead
and try performing class I on separated magnetically separated B-cells
(traditional technique of class II determination) and the method worked.
I would to know if there are other techniques (besides PCR-phenotyping)
to go around this kind of frequently encountered problem.
Thanks
Christian