PE-CY5 BINDING
Mike Keeney (Mike.Keeney@lhsc.on.ca)
Wed, 06 Aug 1997 11:13:18 -0500
There is a letter to the editor in BLOOD , September 1996,
15:88(6):23558-61 by van Vugt, MJ et al which shows that PE-CY5
binds SPECIFICALLY to the Fc(gamma)R1 receptor (CD64). These
experiments using mouse B cells transfected with different human Fc
receptors showed that only FcR1 cells bound PE-CY5. Whole blood
showed much less binding than isolated cells. Their recommendation
was that any antibody that you are using labelled with PE-CY5 be tested
for FcR1 binding.
In our hands blocking with heat inactivated normal human AB serum will
significantly reduce the Fc binding in humans.
Mike Keeney
London Health Sciences Centre
London Ontario Canada