along the same line:
Takizawa, F. Kinet, J.P. Adamczewski, M.
D 1993
T Binding of Phycoerythrin and Its Conjugates to Murine Low Affinity
Receptors for Immunoglobulin-G
%J Journal of Immunological Methods
%C PO Box 211, 1000 AE Amsterdam, Netherlands
%I Elsevier Science Publishers BV
%V 162
%N 2
%P 269-272
%@ 0022-1759
%K Phycoerythrin; Flow Cytometry; Fc Receptor; (Mouse); FC-RECEPTORS; MACROPHAGE
%X Conjugates of R-phycoerythrin are widely used for immunohistochemistry,
especially for two-color flow cytometry. Their use is however limited by
their apparent tendency to bind non-specifically. Using cells transfected
with cDNAs for the murine low affinity receptors for immunoglobulin G
(FcgammaRII and -III) and cells naturally expressing these receptors, we
demonstrate that R-phycoerythrin and its conjugates bind specifically and
inhibitably to FcgammaRII and -III. Immunofluorescence stainings of cells
bearing these receptors, such as macrophages, monocytes, neutrophils, mast
cells, subsets of T cells, and natural killer cells, may therefore not
reflect the binding of antibody to antigen, but rather the binding of
R-phycoerythrin to the receptors.
%W M Adamczewski, Niaid, Molec Allergy & Immunol Sect, Twinbrook II, RM
108, 12441 Parklawn Dr, Rockville, MD 20852
-- ============================================================================== Thomas Delohery |Internet: t-delohery@ski.mskcc.org Supervisor, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================