On Sun, 3 Aug 1997 kcdol@samsung.co.kr wrote:
>
>
> Dear flowers
>
> Have you ever experienced TdT positive AML M3?
>
> The patient was in his 30s and the bone marrow
> was clouded with typical M3 promyelocytes.
> They expressed surface antigen of CD13+and CD33+ without
> HLA-DR.
> All lymphoid lineage markers were negative.
> Our protocol was as follows;
> 1. Mononuclar cell separation with Ficoll-Hypaque
> 2. Wash with 1%BSA-PBS once
> 3. Permeabilization with FACS Brand Lysing Solution
> 4. Wash with 1%BSA-PBS once
> 5. Incubation with anti-TdT MAb
> 6. Wash with 1%BSA-PBS once
> 7. Data aqusition and analysis with FACSort and HP340
> computer system
>
> Autofluorescence without anti-TdT MAb was used as negative
> control and MOLT-3 cell line was used as positive control.
>
> About over 90 percent of promyelocytes were nuclear TdT
> positive.
>
> What's wrong or was it real?
>
> Chang-Seok Ki, MD
> Dept. of Clinical Pathology
> Samsung Medical Center
>
>