Doxorubicin retention assay

Elizabeth Paietta (paietta@aecom.yu.edu)
Mon, 4 Aug 1997 10:23:13 -0400 (EDT)

Hi, I would appreciate any help that I can get on doxorubicin retention
assays. I am performing rhodamine efflux assays routinely but I can't get
the doxo retention assay going. I get excellent uptake but then the doxo
stays within the cells even over a 24 hour period. I have tried various
incubation temperatures and I am using P-glycoprotein positive cells by
rhodamine efflux assay. Any ideas?
Thanks,
E. Paietta