Re: Doxorubicin retention assay

Anna Porwit-MacDonald (anpo@mb.ks.se)
Wed, 6 Aug 1997 10:20:31 +0200

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Elizabeth,
One of our PhD students works with Daunorubicin efflux assay on AML cells
and she has noticed that after about 1h there is a "steady state" which
means that she has to centrifuge cells and change medium again to get more
DNR out. Maybe that is the case with Doxo as well. Just a thought.
Best wishes
Anna Porwit-MacDonald
Haematopathology Lab.
Karolinska Hospital, Stockholm

>Hi, I would appreciate any help that I can get on doxorubicin retention
>assays. I am performing rhodamine efflux assays routinely but I can't get
>the doxo retention assay going. I get excellent uptake but then the doxo
>stays within the cells even over a 24 hour period. I have tried various
>incubation temperatures and I am using P-glycoprotein positive cells by
>rhodamine efflux assay. Any ideas?
>Thanks,
>E. Paietta
>
>
>

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