Re: Viability and Turbo Sort

Altterrain@aol.com
Wed, 30 Jul 1997 18:06:27 -0400 (EDT)

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i have had success with mouse bone marrow. the cell were first lineage
depleted using primary antibodies and a dynabead secondary. the cells were
then sorted for Sca1 positives by our very capable flow operator, kathy
mckinnon (you're welcome kathy, you can mail me the check) on a FACS Vantage
with TurboSort. i believe they were sorted at 30 psi. the Sca1+ cells were
used in our soft agar colony cultures with excellent results.

-brian white
human genome sciences
rockville, md
brian_white@hgsi.com
301-309-8504 x2121

Next message: Dr William Smith: "Cord blood"
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Maybe in reply to: Claude Cantin: "Viability and Turbo Sort"
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