A few small additions to Mario's note. First, annexin V biotin is also
commercially available and indirect staining may be easier than trying to =
do a
conjugate. Second, in my experience annexin V staining will not survive =
PFA
fixation unless some divalent cation (calcium) is present in the fixative =
(and
any wash steps!). I have only used bovine annexin V, but I assume that =
this
will be true of human recombinant annexin V also.
Tom Frey
We haven't specifically tried GFP with annexin, but there is no reason =
for this
not to work--we often use GFP with a wide variety of fluorochromes, like =
PE,
Cy5PE, TR, APC, etc; annexin V can be conjugated to any of these to be =
used in
combination with GFP. If you have the annexin V protein (it can be =
obtained
both commercially and in collaboration with some scientists producing
recombinant versions), conjugation instructions can be found at
"http://cmgm.stanford.edu/~roederer/abcon/".
BTW, the annexin V staining survives 0.5% paraformaldehyde fixation just =
fine.
mr
> Has anyone tried this combination? Or are there any other
> apoptosis markers that would work with GFP?