Re: Methods for sorting viable X and Y sperm

Michael H. Fox (mfox@cvmbs.colostate.edu)
Tue, 17 Jun 1997 10:21:22 -0400 (EDT)

Using an orienting flow nozzle and staining for DNA content using
Hoechst 33342 is the only reliable way to sort viable sexed sperm.
While the idea of using oligos makes sense, the problem is that you
have to kill the sperm to get the oligos into the DNA. No one to my
knowledge has been able to come up with any specific surface markers
that could be used to distinguish X from Y sperm without having to
fix the sperm.

Mike Fox

On Mon, 16 Jun 97 11:56:27 -0600 CHELACK@admin3.usask.ca wrote:

>
> What kind of methods are being used to sort viable X and sperm in particular
> bull sperm. A graduate student has brought me some older literature
> describing sorting on differences in DNA content using specialized adaptors
> to maintain consistent orientation of the sperm as they pass through the flow
> cell. Is it possible to use FITC oligos to specific regions of the Y
> chromosome to accomplish this? I have sent the graduate student back to search
> out more on this possibility, but am wondering if anyone out there is already
> doing this or has found it to be impossible, or is using another even more
> elegant method? The graduate student is quite keen and quite green so I am
> trying to make sure he isn't biting off more than he can chew.
>
>
> regards
>
>
> BJC - Brian

Mike Fox
Chairman, Cell and Molecular Biology Graduate Program
Dept. of Radiological Health Sciences mfox@cvmbs.colostate.edu
Colorado State University Ph: (970)491-7618
Ft. Collins, CO 80523 USA Fax:(970)491-0623
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