Re: Identification of Eosinophils in Flow Cytometry
R Barclay (Robin_Barclay@compuserve.com)
Tue, 17 Jun 1997 18:58:55 -0400
Eosinophils are readily identifiable in most leucocyte preps. They run as
a discrete subpopulation (on a BD FACSort) in a forward-scatter (X) versus
side-scatter plot (Y), above the neutrophils and maybe just slightly behind
them (don't turn the side-scatter gain too high, or you'll lose them out
the top of your plot). Also, if you draw a gate around them and look at
this in any fluorescence channel you'll find them to be more
autofluorescent than other leucocytes.
Robin Barclay
BTS Edinburgh