Re: INDO-1 Calibration

Michael H. Fox (mfox@cvmbs.colostate.edu)
Tue, 17 Jun 1997 10:19:04 -0400 (EDT)

Molecular Probes sells kits to calibrate intracellular calcium. It
is somewhat complicate to do by making up your own buffers, but it
can be done. It might be well worth it to just buy a kit, though,
and more reliable.

On Mon, 16 Jun 97 8:27:13 -0600 HANDLEY@sorter.dfci.harvard.edu wrote:

>
> Hello Everyone!
>
> Does anyone know if there is a kit available to help calibrate a flow cytometer
> for calcium flux using Indo-1? I have a researcher who wants to be able to
> compare values between experiements, and I have suggested using ionophore
> and EGTA to help with this, but he seems reluctant to do all the calculations
> involved. Any suggestions?
>
> Thanks,
> Maris
>
> Maris Handley
> Dana-Farber Cancer Institute
> Boston, Ma 02115
> (617)632-3179
>
> handley@sorter.dfci.harvard.edu
>

Mike Fox
Chairman, Cell and Molecular Biology Graduate Program
Dept. of Radiological Health Sciences mfox@cvmbs.colostate.edu
Colorado State University Ph: (970)491-7618
Ft. Collins, CO 80523 USA Fax:(970)491-0623
====================================