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Here are some references on what we originally callled "Single Organelle
Flow Analysis" (SOFA):
R. F. Murphy (1985). Analysis and Isolation of Endocytic
Vesicles by Flow Cytometry and Sorting: Demonstration of
Three Kinetically Distinct Compartments Involved in Fluid-
Phase Endocytosis. Proc. Natl. Acad. Sci. USA 82:8523-8526.
R. F. Murphy and M. Roederer (1986). Flow Cytometric Analysis of
Endocytic Pathways. In: Applications of Fluorescence in the
Biomedical Sciences, D. L. Taylor, A. S. Waggoner, R. F. Murphy,
F. Lanni, R. Birge (eds.), Alan R. Liss, Inc., New York, pp. 545-566.
R. F. Murphy (1988). Processing of Endocytosed Material.
Adv. Cell Biol. 2:159-180.
R. B. Wilson and R. F. Murphy (1989). Flow Cytometric Analysis of
Endocytic Compartments. Methods Cell Biol. 31:293-317.
R. F. Murphy, M. Roederer, D. M. Sipe, C. Cain, and R. Bowser (1989).
Determination of the Biochemical Characteristics of Endocytic
Compartments by Flow Cytometric and Fluorometric Analysis of Cells and
Organelles. In: Flow Cytometry: Advanced Research and Clinical
Applications, Volume II, A. Yen (ed.), CRC Press, Boca Raton,
FL, pp. 221-254.
Zeidel, M.L., T. Hammond, B. Botelho, and H. W. Harris, Jr. 1992.
Functional and structural characterization of endosomes from toad bladder
epithelial cells. Am. J. Physiol. 263:F62-F76.
Hammond, T.G., D. J. Morre, H.W. Harris, and M. L. Zeidel. 1993.
Isolation of highly purified, functional endosomes from toad urinary
bladder. Biochem. J. 295:471-476.
Harris, H.W., M. L. Zeidel, I. Jo, and T.G. Hammond. 1994.
Characterization of purified endosomes containing the antidiuretic
hormone-sensitive water channel from rat renal papilla. J. Biol. Chem.
269:11993-12000.
Hope this helps.
Bob Murphy
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
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Phone: (765)-494-0757;
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