PI Staining of Yeast AND Decreased Signal

Wed, 2 Oct 1996 12:06:54 +0100 (BST)

Like Mark Miller, my flow experience is mostly with mammalian cells, but was
recently asked to make some cell cycle studies with C.albicans.

We found the methods recommended for PI staining of S.cerevisiae in...

Methods in Cell Biology Volume 42
Flow Cytometry (Second Edition) Part B
Eds Darzynkiewicz et al
Chapter 27

...gave excellent results. This (and volume 41) are top texts!

Also of course, we saw an unexpected, and significant, drop in the PI signal
under certain culture conditions as referred to recently by Bruce Milthorpe and
Rob Chervenak (although I missed that mail myself). We find the suggestion that
supercondensation / repacking of DNA would account for this drop in fluorescence
due to some sort of steric hinderance to intercalation to be reasonable but are
surprised by the apparent absence of reports or analysis of this phenomena in
the literature, and not knowing much about yeast, are a little bit at a

Can anyone shine some more light on this subject?

Justin Lamb
Cell Pathology Laboratory
University Medical School
Scotland UK

F: +44 1224 663002
E: j.lamb@abdn.ac.uk

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