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Please send your message to the cytometry list so that you get a
greater audience....
cytometry@flowcyt.cyto.purdue.edu
regards
paul
From Nan.Lin-2@tc.umn.edu
Wed Nov 27 17:47:30 1996
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From: Nan Lin <Nan.Lin-2@tc.umn.edu>
To: cyto-inbox
Subject: Annexin V
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Hi Paul:
I read the paper buy van Engeland on Cytometry 24, 131-9. I found it very
fancinating! My prof and I are thinking about using Annexin V on our
project to determine apoptosis. I have some questions about that after
reading the paper. They mentioned that using trypsin can cause increasing
PS exposure thus bring false positive results. They recommended mechanical
scratch. We are working on MCF-7 cells, they are adhensive. When we add
estradial as positive controls, they cells become even more sticky. If we
can't use trypsin, how do we make single-cell suspension???
If you or any one can help, we'd really appreciate. Thanks and have a nice
thanksgiving!!
Nan Lin, PhD student
Toxicology program, Univ of Minnesota
J.Paul Robinson, Purdue University Cytometry Labs
Professor of Immunopharmacology
robinson@flowcyt.cyto.purdue.edu PH:317-494 6449 FAX:317-494 0517
web http://www.cyto.purdue.ed
~~~~~~~~~~~~~~~ Forwarded message ends here ~~~~~~~~~~~~~~~
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web