Fluorescence quenching by HCl

Charles Kuszynski (CKUSZYNS@MAIL.UNMC.EDU)
Tue, 10 Dec 1996 10:43:00 -0600

We have located a protocol for examining the internalization of
fluorescent ligands by acid washing of the external fluorescein. The
problem is the reference cited below does not contain any methodology
for the acid wash step. Has anyone out there done a procedure similar
to this?

Ref: Van Epps et al.
Jounal of Immunology
144:1062 ff.
Feb. 1990

Thanks

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