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PE/Cy5 reagents tend to be brighter, at least in my experience. The big
selling point of PerCP is that it does not require significant FL2-%FL3
compensation. This seems a silly claim to make in that the FL3-%FL2
compensation is always many times greater than the FL2-%FL3, i.e. it
takes a lot more comp to get the PE signal out of my FL3, than to get
the PE/Cy5 out of my FL2 AND this larger compensation is independent of
which FL3 reagent you use. I used a CD4 PE/Cy5 last week which required
a 2% comp.
PerCP has been used on stream in air sorters with generally poor
results, whereas PE/Cy5 works fine.
A final note: Unlike FITC or PE, PE/Cy5 is not purchased from a
manufacturer; each company's chemist is making their own "homemade"
PE/Cy5 conjugate. Thus each will have slightly different compensations
required.
Now when is somebody gonna make me an APC/Cy7 streptavidin conjugate???
Calman Prussin
NIAID/ NIH
>----------
>From: steven micko
>Sent: Wednesday, September 4, 1996 10:02
>To: Cytometry Mailing List
>Subject: CyChrome & PerCP
>
>
>
> Can anyone give me information on the chemical structure (in a general
> way), excitation/emission information, and stability information on the
> two fluorochromes, CyChrome (Pharmingen) and PerCP (B-D)? I'm not
> interested in violating anybody's proprietary information. I just want to
> know what I'm dealing with. So far all I have been able to get is
> marketing stuff (i.e. ours is better than theirs).
>
> Is there anything in the literature?
>
> Thanks in advance.
>
> Steve Micko
> Emory Hospital
> Atlanta
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web