 |
 |
 |
 |
 |
an investigator here has been studying the effect of various peptides on er
negative cells (monocytes and dendritic cells).
our question is whether using ionomycin (as our positive control/at various
concentrations) should the ratio of violet to blue return to near baseline
after the influx of calcium?
we are loading with indo-1 and are using an argon ion laser tuned to UV.
we get increases in ratio of violet to blue of about 4:1 after stimulation but
this stays high even for 10 minutes.
this is my first time doing calcium flux experiments and i would be very
grateful to hear from anyone with some comments.
thank you very much.
frank isdell
flow cytometry
the rockefeller university
1230 york ave
ny 10021
tel 212-327-7657
fax 212-327-8875
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web