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>I'm looking for a comercial source of the 633nm excitable dye
>carboxy-naphthol-fluorescein-diacetate.
That was originally made by Molecular Probes; if they don't have it, I don't
know who else would.
> Also I would be interested to
>hear of any other red-excitable dyes that can be used for viability
>measurements - particularly in animal cells
Most people would consider TO-PRO-3 and TOTO-3 usable for dye exclusion
viability tests in animal cells, although these dyes do tend to get in
slowly. With your experience, you might want to look at the oxonol
DiTBAC4(5), available from Molecular Probes; while this is nominally a
membrane potential stain, it will stain the intracellular contents of cells
with damaged membranes, making them a lot brighter than cells with intact
membranes and no membrane potential.
-Howard
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web