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We are using a BD FACScan. We are currently using Gentrak lyse/fix, although I
think we're going to try the BD stuff.
I'm looking simply at what changes occur in the basic lineages (mono, gran,
lymph) when the mice are treated with novel proteins. I am using a three tube
panel: isotype control, B220/CD3 and CD14/Gr.1 (two color).
I'm more familiar with human LWB; the mouse FSC v. SSC looks similar but the
monocytes aren't as clearly separated--is this usually the case with mice?
Any mouse flow hints would be great,
Thanks,
Jeff Carrell
e-mail:
jeff_carrell@hgsi.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web