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Two things spring to mind:
When I did some UV work on a FACS 440 some years ago, I found the side
scatter to be MUCH lower sensitivity than with visible laser; I think that
UV light is not scattered as much, and the PMT was less sensitive to UV.
I did not get ANY UV side scatter until I removed the 488 bandpass filter
from the side scatter detector! (took me at least a week to think of it...)
Good Luck, Joseph.
Joseph Webster (O.I.C. Flow Cytometry & Communications) ===
Centenary Institute of cancer Medicine & Cell Biology / \
Locked Bag No.6 || o o ||
Newtown, NSW 2042 || | ||
AUSTRALIA. | \___/ |
Ph: 61-2-565-6110 \ /
Fax: 61-2-565-6101 |||||
E-Mail: J.Webster@centenary.usyd.edu.au
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web