 |
 |
 |
 |
 |
My Elite has a dichroic filter that excludes the UV line from the side
scatter detector. To get wavelength independent side scatter, I made an
angled reflector using a glass cover slip glued into a spare filter holder.
This directs a small proportion of the light into the side scatter detector
and lets the rest through to the other detectors. It works just fine and
also gives a side scatter signal with the red HeNe laser.
I assume that some arrangement like this is possible on most machines.
The Elite has a 488nm filter ahead of the forward scatter detector. This
must be removed when working at other wavelengths.
Good Luck,
Dean R. Hewish, Cell Biologist & Flow Cytometrist. ( and Amateur Malacologist)
CSIRO Biomolecular Engineering, 343 Royal Parade, Parkville, 3052,
Victoria Australia.
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web