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> Hello fellow cytometrists,
>
> One of our researchers wants to "measure apoptosis in cell
> suspensions made from solid tumours fixed in 70% ethanol (or other
> fixatives as apprpriate)."
>
> Can anyone one point us in the right direction?
>
> Thanking you for your time and trouble ....
>
I presume he means an end-labelling technique with TdT and FITC labelled
d-UTP. We have been doing this for a while and it seems fairly reliable.
Try Bromidge et al, Cytometry 20: 257-260, 1995.
If you wish I have a working protocol which I can post.
Eric Miller, ICRF Medical Oncology Unit, Edinburgh UK
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web