Re: PNA+ Controls

Stephen G. Kayes (kayes@sungcg.usouthal.edu)
Wed, 6 Sep 1995 10:00:26 -0500 (CDT)

I was not aware that the thymus, human or otherwise, had (a) germinal
centers. The pale staining areas of lymphocytes are thought to be
predominantly dividing medium to large T cells or their precursors. I am
not saying that there might not be B cells in the thymus, but
histologically speaking, there are no germinal centers in thymus. The
pale staining areas are properly called the thymic medula. The
functional significance here is that true germinal centers are
collections of B cells aggregated on a meshwork of reticular fibers. The
thymus has no such fiber population. Instead, the epithelial-reticular
cells extend finely attenuated processes from one cell to another to form
the meshwork between the thymic lymphocytes. It is assumed that T cell
selective adhesion molecules are responsible for the aggregation of T
cells in the appropriate stage of development (ie showing the appropriate
lineage specific markers on their surface and acting as ligand for the
the adhesion molecules) in and among the epithelial reticular cells.

Steve Kayes

On Tue, 5 Sep 1995, Jonni Moore wrote:

>
> We have a user who wants to sort germinal center B cells from human thymus
> based on CD20+/peanut agglutinin(PNA)+ criteria. Does anyone know what an
> appropriate negative control for the PNA would be. Normal isotype would
> not work since it is not an immunoglobulin. We are interested in sorting
> both positive and negative populations and need good controls since the
> negative population may be <2%.
>
> Thanks for any suggestions anyone might have!
> Jonni
>
>
>
> Jonni S. Moore, Ph.D.
> Director, Cancer Center Flow Cytometry and Cell Sorting Facility
> University of Pennsylvania School of Medicine
> 297 John Morgan Bldg
> Philadelphia, PA 19104-6082
> Phone: 215-898-6853
> FAX: 215-898-4227
> e-mail: moorej@a1.mscf.upenn.edu
>
>
>

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