 |
 |
 |
 |
 |
The problem is with any machine I have experience of (Coulter and BD)
Going faster DOES reduce purity to a significant extent, co-incidence
abort or not. I agree that sorting completely negative cells is much
easier, but they are even easier to select in greater numbers, faster and
with a similar purity using magnetic beads. For functional studies
beads have the same benefit of minimal biological modification.
Flow sorting is most useful in my opinion for sorting the difficult
stuff
Have fun
Mike
...........................................................
Mike Salmon
Department of Rheumatology
The Medical School
University of Birmingham
Birmingham B15 2TT
United Kingdom
Tel: 44 (0)121 414 6780
Fax: 44 (0)121 414 6794
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web