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I am also contemplating sorting cells for extraction of RNA. However, my
cells (HIV+ human T cells) are fixed in paraformaldehyde before I get them.
Has anyone any information as to the possibility of extracting mRNA from
such fixed cells, with a view to performing RT-PCR?
Margaret A. Cooley, PhD
Centre for Immunology
St Vincent's Hospital
Darlinghurst NSW 2010 AUSTRALIA
Ph: 61-2-3617700; Fax: 61-2-3612391.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web