COULTER PCNA-FITC

COULTER™ PCNA-FITC

COULTER PCNA-FITC is a monoclonal antibody directed against Proliferating Cell Nuclear Antigen. PCNA is closely associated with the sites of DNA replication in the cell nucleus. The level of PCNA synthesis directly correlates with cellular proliferation, specifically DNA synthesis during cell cycle. COULTER PCNA-FITC is available only for fundamental research and is not for clinical diagnostic use.

Dot Density Plot (PCNA-FITC vs. propidium iodide) A propidium iodide stained isotype control was prepared and anlyzed. Subsequently, MOLT 4 cells stained with COULTER PCNA-FITC/propidium iodide were analyzed. The majority of the cells were included in the gate, then imported into a FL3 vs. FL3P histogram to enable doublet discrimination. After eliminating doublets by drawing an additional gate, the data is viewed in a FITC/PI scatter plot. (Note the increase in cells in S phase and G2/M phase (compared to the control).)

Example Research Areas: Cellular proliferation studies Bolton, W. et al. Cytometry, 1992, 13:117-126. Bolton, W. et al. Cytometry, 1994, 17:66-74. Marker for S-phase cells as a replacement for 3H-thymidine pulse labelling Galand, P. & Degraef, C. Cell Tissue Kinet, 1989, 22:383. Identification of blast cells in leukemia Takasaki, Y., et al. JNCL, 1984, 73:655. Selective detection of proliferating cells in solid tumors and transformed cells Mikulka, W. & Bolton, W., Cytometry, 1994, 17:246-257. Indicator of disease activity in active lupus erythematosus Murashima, A., J Clin Immunol, 1990, 10:28.

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Single Parameter DNA Histogram

Additional analysis of DNA content of the same MOLT 4 cells is shown above. The cursor represents S and G₂/M cell cycle phases.

Molecular Orientation Proliferating Cell Nuclear Antigen (PCNA) is a nuclear associated intracellular marker. PCNA is tightly associated with DNA polymerase delta. Cell Cycle PCNA peak expression occurs in late G1, S and G2/M cell cycle phases.

COULTER (TM) PCNA-FITC Specificity PCNA, formally called cyclin, is a 36kD intranuclear polypeptide found in both normal and transformed cells. It is highly conserved in mammals. PCNA is the auxiliary protein to DNA polymerase-delta (associated with sites of DNA replication). The level of PCNA synthesis correlates with rates of cellular proliferation and DNA synthesis. Elevated levels of PCNA appear in the late G1 phase of cell cycle, maximizing during S phase and declining during G2and M phases. Product Description COULTER PCNA-FITC is in liquid form and is directly conjugated. Concentration: 1µg/10 µL Vial Contents: 0.5 mL Isotype: IgG1 Part Number: 6607002 Chromosome 20 The PCNA gene is located on human chromosome 20.

For Research Use Only. Not for Use in Diagnostic Procedures.

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© Coulter International Corporation, 1996. All rights reserved. Copyrights and Trademarks

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu