TUNNEL POS. CONTROL
Angel_Delgado@ftdetrck-ccmail.army.mil
Fri, 12 Jan 96 13:38:35 EST
I have been using the In Situ Cell Death Apoptosis kit from Boehringer
Mannheim for a while now. It seems to work really well. For a
positive control I use 10 units of DNase 1 Amplification grade from
GibcoBRL (part# Y02050). I add the DNase after the cells have been
treated, fixed, and permeabilized. I have been getting approximate
85% positive population. I am using human granulocytes in this study,
but I do not believe DNase 1 is bias to certain DNA. Good luck!!!
NOTE: Apoptosis should always be verified by morphology according to
its cell type.
Angel Delgado
Immunologist
US Army Institute of Surgical Research
Fort Sam Houston
San Antonio, Texas 78234
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
