Expression of Annexin on Activated platelets

colette charland (ccharlan@zoo.uvm.edu)
Fri, 8 Nov 1996 10:07:05 -0500

Greetings from the University of Vermont

An investigator wants to look at increased expression of annexin
fitc on activated platelets. Is there any way of differentiating an
increase in the intensity of the signal due to upregulation on such
platelets from increased signalling due to the beginning of aggregation. It
appears that the higher level signalling also demonstrates increasing FS and
SS.
I would like to know whether measuring total fluorescence, as
opposed to looking at the mean intensity of the signal is the answer. We
have an EPICS Elite, if anyone has any insights, they would be greatly
appreciated.

Colette Charland

Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu