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I need some advices regarding the use of ModFit S/W.
A colleagues of mine is interested in studying the effect of various
anti-sense oligo's on cell cycle progression of synchronized HeLa cells. He
have tried to use BD's ModFit s/w to analyze the
data and did not get good reports with some of the data files. I studied
some of the graphs and found that bad reports are usually derived from
non-standarized cell cycle distribution. For example, one set of data can
have 10% G0/G1, 80% S and 10% G2/M. The s/w scans three peaks and take the
second S peak as G2/M peak. Another example will be a set of data with 20%
G0/G1 and 80% S and invisible G2/M. The s/w will scan and see only one
peak.
I used to work with the earlier vesion (CellFit) and my only
experience with ModFit is with standard cell cycle data. It appears that
the CellFit S/W does a better job in these type of analysis. I had
used CellFit for similar type of analysis and it generated better reports.
We would like to know if ModFit is fitted for such an analysis, and if
yes, how to get around the proplems we had? We would try other s/w if
modfit is not adequate for such analysis. Would anyone suggest a good
Mac-s/w for us? (We have a FACScan and Macintosh Quadra 650) Thank you in
advance.
Regards
Austin Lin
austin63@ms12.hinet.net
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