Re: Increasing Cytoplasmic fluorescence

Deborah Berglund (umbbd@gemini.oscs.montana.edu)
Wed, 6 Nov 1996 12:15:28 -0700 (MST)

Hi,

I used to see similar increases when staining with FDA, as it leaked out
of the cells the medium surrounding the cells became fluorescent,
therefore increasing the signals.

We timed the analyses precisely so that each sample was analyzed exactly
5 minutes after staining.

Deb Berglund
Montana State University

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