Re: PMN Markers
Howard Shapiro (hms@shapirolab.com)
Wed, 30 Oct 1996 09:02:55 -0500 (EST)
One of the problems with basophils is that many treatments applied to blood
samples, e.g., ammonium chloride lysing solution, will cause them to
degranulate, which is one reason why they generally land in the area of the
"lymphocyte" cluster on a plot of forward vs. orthogonal scatter. I was
under the impression that depolarized scatter could discriminate basophils
from other cells, as is the case with lymphocytes, but Leon Terstappen and
Bart DeGrooth should know best about that.
-Howard
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