Moab to CD40L

Ronald L. Rabin, M.D. (rr84g@nih.gov)
Fri, 11 Oct 1996 14:09:44 -0400

A little bit of confusion regarding my reply to the scientist seeking a
monoclonal to human CD40L. I have Pharmingen's PE conjugate, and have used
it on cells after 3 days stimulation. They express no CD40L at this time
(not surprising), and thus, I cannot assess the quality of this antibody.
A colleague of mine here at NIH has used Pharmingen's unconjugated mAb on
PBMC 6 hours after stimulation with PMA, and he said that it works quite
well.

Now let's take a deep breath, and have a nice weekend.

ron

Ronald L. Rabin, M.D.
Clinical Associate
Cytokine Biology Unit
LCI/NIAID NIH
phone: (301) 402-4910
fax: (301) 496-7383
e-mail: rr84g@nih.gov

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