Re: Calcium data analysis

Ronald L. Rabin, M.D. (rr84g@nih.gov)
Thu, 10 Oct 1996 09:32:24 +0800

>Hello Flowers,
> I would like to know if anyone knows of a program that I may use to
>analyze calcium data collected on a FacStar plus with a HP computer
>system. I previously used COTFIT and KINPRO programs on the Vax system.
> I also have a MAC, but there is know time parameter in the Cellquest
>software. I would like to be able to retrieve data points, and create
>a plot. I am currently looking at a demo disk of Multitime. Any
>suggestions are appreciated.
>
>
>
>Carolyn D. Dawson
>Centers for Disease Control, MS A-25
>1600 Clifton Road
>Atlanta, GA 30333
>404-639-2721

Cellquest does have a time parameter that can used with ratiometric
analyisis. Are you copying your files as FCS files and using BD's
"FACSconvert?" You may want to give BD a call.

Ronald L. Rabin, M.D.
Clinical Associate
Cytokine Biology Unit
LCI/NIAID NIH
phone: (301) 492-4910
fax: (301) 496-7383
e-mail: rr84g@nih.gov


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu