CD34+ selecting and culture

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We are trying to separate CD34+ cells using MACS. After separating we
would like to culture them, but they do not grow. What are the optimal
separating methods (using MACS) and what are the optimal growth
conditions after separating.
Conditions have to be sterile.

Thank you !

Return e-mail: kalmanm@rosi.szbk.u-szeged.hu

Arpad Farkas
Szent-Gyorgyi Albert Medical University
Szeged, Hungary

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu