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I am in the process of budget writing, and would like to know what kind of
experience the network has with magnetic cell separation devices. We are
considering the addition of this type of service to our facility. I
have literature on the MACS (Miltenyi), Dynabeads (Dynal) and Biomag
(Advanced Magnetics). Our primary use would be separation of tissue (blood,
etc..) derived animal cells and culture derived human cells using goat
anti-mouse coated beads as a positive selection. The MACS beads are quite a
bit smaller (50nm) than the other brands and require more powerful magnets.
Is there any evidence that the higher magnetic field is detrimental to the
cells? Are the beads known to "activate" the cells and do they need to be
removed? We are probably looking at separations of 10-100 million cells.
What level of purity is observed? Is it best to use this hand-in-hand as a
pre-enrichment with FACS providing the end-product?
Can magnetic beads be used to separate nucleic acids based upon hybridization
to biotinylated probes? Would the bead size/number of beads bound (i.e. size
of probe) prove to be the limiting factor in this case? Food for though
anyway.
My summary on the responses to my (ancient history) request on chromosome
sorting will be forthcoming. I would be willing to share the response I get to
the above questions, if the response-level is high.
Thanks,
--- ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Kristi R. Harkins, Facility Director <> Office: 515/294-2472 Cell & Hybridoma Facility <> Lab: 515/294-8504 Iowa State University <> FAX: 515/294-0453 1104 Molecular Biology Bldg. <> E-mail:kharkins@iastate.edu Ames, IA 50011 <> xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
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