dye soln. and blank beads as a standard

vanburen%flovax.dnet@rocdec.roc.wayne.edu
Fri, 19 Nov 93 15:28:30 -0500

We have recently started work on an application in need of standards. We
would like a UV-excitable (350-365nm from Ar+ laser), violet-fluorescing
(400-450nm) standard. Most beads are either too bright (FCSC Hoechst, Indo,
and FullSpectrum) or too dim (FCSC Blank). I remember hearing about a
method using a dye solution with blank beads as a trigger source. Does
anyone have a procedure worked up for this method, or remember a source for
one? Any other ideas?

/\/\/\_ Eric Van Buren, vanburen%flovax.dnet@rocdec.roc.wayne.edu
\ \ \ Immunology & Microbiology
\_^_/ Wayne State University


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu