RE: NON-HUMAN PRIMATE FLOW CYTOMETRY

Dawson, Carolyn (cdd1@CIDHIV7.EM.CDC.GOV)
Thu, 30 Sep 93 13:32:00 EST

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REPLY FROM: Dawson, Carolyn
Microsoft Mail v3.0 IPM.Microsoft Mail.Note
From: Rowe, Thomas
To: Dawson, Carolyn
Subject: RE: NON-HUMAN PRIMATE FLOW CYTOMETRY
Date: 1993-09-23 12:16
Priority: R
Message ID: AA476A65
Conversation ID: AA476A65

In response to your question concerning staining of non-human primates for
flow cytometery, Anne Brodie from the Yerkes Regional Primate Center in
Atlanta, Georgia has informed me that the BD reagents Leu3a and Leu2a (anti
CD4 and anti-CD8 respectively) work very well for the Great Apes as well as
Pigtail and Stumptail macaques. However, their Leukogate product does not
work very well. She has informed me that there might be some gating tricks
in order to obtain optimal staining. Please give her a call at
(404)727-7757 for further information.

Good luck,
Thomas Rowe, Centers for Disease Control and Prevention, Retrovirus Diseases
Branch
Atlanta, Georgia

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu