DNA linearity
Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
27 Sep 93 18:53:29 EDT
Has anyone had any trouble with non-linearity during DNA measurements on the
FACScan? We have just suddenly found that, although the FL2-Height and
FL3-Height signals show fluorescence intensity ratios at about exactly 2.0
for doublets and for G1/G2, the FL2-Area gives a ratio significantly greater
than 2.0 for these peaks from the same samples. What are the reasons that
the area of the fluorescence signal might not be linear with intensity? Why
has this happened suddenly? Is this likely to be a fluidics problem or an
electronic one? What can we do about it????
Thanks for any help anyone can offer.
Alice Givan
Alice.L.Givan@dartmouth.edu
NCCC Flow Cytometry Laboratory/Department of Physiology
Dartmouth Medical School
Lebanon, New Hampshire 03756-0001 USA
voice 603-650-7907 fax 603-650-6130
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