Response to John Chan - chromosomes

Scott Cram (cram@telomere.lanl.gov)
Wed, 23 Jun 93 12:41:37 MDT

John, We have successfully analyzed mammalian chromosomes on a BD FACS
Scan analyzer but only with ideal preps. If you are working with a
simple karyotype (euploid Chinese hamster for example) you can resolve
about eight peaks. Filter selection is critical as is sample
concentration and alignment. If you are interested in more details
give me a call (505) 667-2890 or on e-mail: cram@telomere.lanl.gov
Good luck - always happy to see more investigators working with
chromosomes!
Scott Cram
Life Sciences Division
Los Alamos National Laboratory
Los Alamos, NM 87545]


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